UBE2L3 Protein (AA 1-154)

Catalog No. ABIN666841

Recombinant UBE2L3 protein expressed in Escherichia coli (E. coli).

Quick Overview for UBE2L3 Protein (AA 1-154) (ABIN666841)

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-154

Sequence: MAASRRLMKE LEEIRKCGMK NFRNIQVDEA NLLTWQGLIV PDNPPYDKGA FRIEINFPAE YPFKPPKITF KTKIYHPNID EKGQVCLPVI SAENWKPATK TDQVIQSLIA LVNDPQPEHP LRADLAEEYS KDRKKFCKNA EEFTKKYGEK RPVD

Characteristics: Ubc7(Ubiquitin Conjugating enzyme 7) Human Recombinant, expressed in E.coli

Purification: > 95% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 50 mM HEPES buffer ( pH 7.4) containing 150 mM NaCl, 1 mM DTT, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Alternative Name: UbcH7/UBE2L3

Background: Human ubquitin-conjugating enzyme 7 (ubcH7) is a ubiquitin-conjugating enzyme (E2) mediating c-fos degradation, transcription factor NF-kappa B maturation, and human papilloma virus-mediated p53 and Myc protein degradation, in vitro. The ubiquitin-conjugating enzymes (E2s) are essential components of the post-translational protein ubiquitination pathway, mediating the transfer of activated ubiquitin to substrate proteins. The human uBE2L1-uBE2L4 gene could potentially encode different isoforms of the ubcH7. uBE2L3 gene, located at chromosome 22q11. 2, is the only identical family member with introns and encodes a polypeptide sequence identical to that of ubcH7. ubcH7 (154amino acid) was over-expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 17.9 kDa (154aa) confirmed by MALDI-TOF

NCBI Accession: NP_003338